The US has placed Cuba again on a list of state sponsors of terrorism, citing the communist country's backing of Venezuela.
President Donald Trump's administration made the announcement just days before he leaves the White House.
President-elect Joe Biden, who uses office about 20 January, possesses previously said he really wants to improve US-Cuban relations.
Cuba called the approach "political opportunism".
Mr Biden has said he's seeking better ties between your long-term adversaries but Mr Trump's decision will probably hinder a quick repair of relations.
Cuba's put on the list will demand a formal analysis that could take months, analysts say.
The Caribbean island was removed from the list by President Barack Obama in 2015, but Mr Trump has taken a harder line towards the united states.
When explaining the decision, officials cited Cuba's support of Venezuelan head Nicolas Maduro who the US won't recognise.
"With this action, we will once again hold Cuba's government accountable and send a clear message: the Castro regime must end its support pertaining to international terrorism and subversion of US justice," US Secretary of Express Mike Pompeo stated in a statement on Monday.
In response, Cuban Foreign Affairs Minister Bruno Rodriguez tweeted: "We condemn the cynical and hypocritical qualification of Cuba as circumstances sponsor of terrorism, announced by the United States."
In advance of the announcement, House Democrat Gregory Meeks called it "another stunt by President Trump and Pompeo, trying to tie the hands of the incoming Biden administration on the way out the door."
Mr Biden's ideas included allowing Cuban-Us citizens to go to family and send cash.
President Obama normalised relations with Cuba found in 2015. He called the decades-long US work to isolate the united states "a failure".
Since the Cold War era, the US had pursued various plans to undermine Cuba which it saw as an excellent threat.
Cuba now rejoins countries including Iran and North Korea on the set of sponsors of terrorism. The effect on the island nation include extreme limits on foreign purchase.