Barcelona's appeal against Lionel Messi's two-match ban following his sending-off in the Spanish Super Glass last was rejected on Friday, options from the Spanish Football Federation told AFP.
It means the Argentine will never be in a position to play for Barcelona found in Sunday's game away at Elche found in La Liga.
Messi served the initially meet of his suspension seeing as the Catalans won 2-0 in more time away to close to neighbours Cornella, of Spain's third-tier Segunda B division, on Thursday found in the Copa del Rey.
He was sent off late found in more time in Sunday's Super Cup final found in Seville against Athletic Bilbao, which Barcelona lost 3-2.
Messi saw the initially red cards of his Barcelona career, on his 753rd appearance, for lashing out at Athletic's Asier Villalibre.